Project Overview
This project explores gene expression differences between healthy bladder tissue and superficial (early-stage) bladder cancer. Using bioinformatics tools like R, CIBERSORTx, and gene enrichment analysis, our team identified key immune pathways and cell types involved in tumor development and recurrence.
Project
Tools & Techniques: R, T-Tests, Gene Ontology Enrichment, CIBERSORTx, Graphing in R, Data Cleaning & Analysis
Tech Stack
This is the space to introduce the Services section. Briefly describe the types of services offered and highlight any special benefits or features.
Service Name
This is the space to introduce the Services section. Briefly describe the types of services offered and highlight any special benefits or features.
Service Name
Clinical Impact
Early detection biomarkers like HLA-DRA and DOCK10 could support proactive screening, especially in high-risk groups (e.g., cystic fibrosis patients)
Support emerging treatments that restore immune balance
Key Takeaways
Key Findings
Increased Tregs and dendritic cells contribute to immune evasion
Suppression of CD4/CD8 T cells weakens immune memory and tumor response
Identified drug targets: PALBOCICLIB (CDK4/6 inhibitor), AMG900 (Aurora kinase inhibitor)
Data Overview
1836 samples across 233 patients
Analyzed 43,000+ genes with statistical testing (T-tests on 803 genes)
Core Analysis
Differential gene expression between normal tissue and primary BC superficial tumors
Enrichment analysis revealed immune suppression and chronic inflammation pathways
CIBERSORTx used to estimate immune cell types in the tumor microenvironment
Bladder Cancer Gene Expression Analysis
Cystic Fibrosis
HLA-DRA is involved in critical processes linked to cystic fibrosis
Cancer Risk
Individuals with CF are 5-10x more likely to develop colon cancer.
Highlights the need for proactive screening in CF patients.
Corneal Dystrophy:
A condition characterized by recurring structural abnormalities in the cornea.
Known for its recurring nature
Both conditions involve incomplete resolution of the initial damage, leading to a cycle of recurrence.
